Table 2.

Results of in vitro characterization of tailoring enzymes encoded by the bcm cluster. “U” means unidentified products that did not correspond to any product observed in culture supernatants of the S. cinnamoneus strains (Supplementary data sheet 1).

Substrate	Enzyme	Observed products	Relevant Supplementary information
cIL (2)	BcmE	7	Supplementary Fig. S5B for the reaction of BcmE on 2. Supplementary Figs. S11-S12 for NMR data for 7.
cIL (2)	BcmC	U	Supplementary Fig. S5A for the reaction of BcmC on 2.
cIL (2)	BcmG	U	Supplementary Fig. S5C for the reaction of BcmG on 2.
cIL (2)	BcmE + BcmC	7, 6	Supplementary Fig. S6A for the reaction of BcmE + BcmC on 2. Supplementary Figs. S13-S14 for NMR data for 6.
cIL (2)	BcmE + BcmG	7, U	Supplementary Fig. S6B for the reaction of BcmE + BcmG on 2.
cIL (2)	BcmE + BcmC + BcmG	7, 6, 5, U	Supplementary Fig. S6C for the reaction of BcmE + BcmC + BcmG on 2. Supplementary Figs. S15-S16 for NMR data for 5. Supplementary table S6 for stereochemistry data for 5.
cIL (2)	BcmE + BcmC + BcmG + BcmB	7, 6, 5, 4, c2, U	Supplementary Fig. S6D for the reaction of BcmE + BcmC + BcmG+BcmB on 2. Supplementary Figs. S17-S18 for NMR data for 4. Supplementary table S7 for stereochemistry data for 4.
4	BcmD	3	Supplementary Fig. S7 for the reaction of BcmD on 4.
3	BcmF	bicyclomycin (1)	Supplementary Fig. S8 for the reaction of BcmF on 3.