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. 2019 Dec 27;9:20150. doi: 10.1038/s41598-019-56772-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fabricated microfluidic disk chip. (A) The assembled fluidic placed on the heater stage. Inset: microfluidic disk chip. (B) The disk consists of four layers of PDMS with PMMA bottom layer as support. (C) The ring channel served as the reaction chamber. Expanded image shows the height depression that prevents the (D) microbeads carrying the capture antibodies from escaping the main channel.