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. 2019 Dec 27;9:19934. doi: 10.1038/s41598-019-56157-9

Figure 8.

Figure 8

Representative chromatogram of the N-terminal metabolites of [Pyr1]apelin-13 identified in human plasma. (A) [Pyr1]apelin-13(6–13) with retention time of 8.01 minutes, (B) [Pyr1]apelin-13(7–13) with retention time of 8.08 minutes, (C) [Pyr1]apelin-13(8–13) with retention time of 9.35 minutes, (D) [Pyr1]apelin-13(10–13) with retention time of 10.45 minutes, (E) [Pyr1]apelin-13(11–13) with retention time of 9.32 minutes. The mass accuracy of the experimentally acquired monoisotopic peak was calculated for each potential metabolite, and is included along with the 13C isotopic cluster for each peptide with their corresponding chromatogram.