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. 2019 Dec 27;9:20021. doi: 10.1038/s41598-019-56277-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ONECUT2 modulates histone methylation through PRC2. (A) Western Blot of whole cell lysates from A549-ONECUT2 and A549-E.V. cells using indicated antibodies. The full-length blot is presented in Supplementary Fig. 5. (B) Heatmap of H3K27me3 and EZH2 log₂ fold change signal (A549-ONECUT2 versus ΔDBD) around centers of H3K27me3 loss regions. (C) Plot of average EZH2 log₂ fold change by ChIP-Seq (A549-ONECUT2 versus ΔDBD) around activating and repressive ONECUT2 peaks. (D,E) GSEA of ONECUT2-activated genes (D) and ONECUT2-upregulated genes (E) on gene list pre-ranked by log₂FC after GSK126 treatment. (F) GSEA of GSK126 upregulation gene set on gene list pre-ranked based on log₂FC (KPE versus KP) (Kras^G12D/+; Trp53^fl/fl; Eed^fl/fl versus Kras^G12D/+; Trp53^fl/fl). (G,H) GSEA of ONECUT2-activated genes(G) and ONECUT2-upregulated genes(H) on gene list pre-ranked by log₂FC (KPE versus KE).