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. 2019 Dec 27;9:20192. doi: 10.1038/s41598-019-56723-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Estradiol and leptin alter weight gain and food intake in adult female ob/ob and lean control mice. (A) Average weight, (B) percent weight gain and (C) food intake (2 mice/cage) of heterozygote controls (Het) E2 (n = 13), Het Veh (n = 12), ob/ob E2 (n = 8) and ob/ob Veh (n = 7) with arrow indicating the start of high fat diet. The effects of genotype on average weight (A) and food intake (C) were present on all days. Days with effects of E2 are denoted by *(p < 0.05; ANOVA). Error bars indicate ± SEM.