Table 2.
Suitability ranking, based on the geNorm program, of the eight candidate genes as potential reference genes for qPCR experiments involving leaves and roots of ramie plants under different treatments.
| Tissue | Rank | Total | Abiotic | Hormone | P. vexans | SA | BTH | JA | ETH | GA3 | Heat | Cold | NA | Cd | PEG |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Leaf | 1 |
ACT1 (1.13) |
CYP2 (0.62) |
18S (0.84) |
F-box (0.54) |
EF-1a (0.11) |
18S (0.16) |
ACT1 (0.15) |
UBQ (0.18) |
18S (0.42) |
18S (0.10) |
18S (0.21) |
CYP2 (0.29) |
CYP2 (0.40) |
ACT1 (0.51) |
| 2 |
UBQ (1.13) |
F-box (0.62) |
ACT1 (0.84) |
UBQ (0.54) |
GAPDH (0.11) |
ACT1 (0.16) |
UBQ (0.15) |
TUB (0.18) |
F-box (0.42) |
CYP2 (0.10) |
TUB (0.21) |
UBQ (0.29) |
F-box (0.40) |
GAPDH (0.51) |
|
| 3 |
GAPDH (1.71) |
ACT1 (1.73) |
UBQ (0.99) |
EF-1a (0.71) |
CYP2 (0.14) |
F-box (0.18) |
F-box (0.28) |
CYP2 (0.21) |
ACT1 (0.51) |
UBQ (0.48) |
EF-1a (0.32) |
F-box (0.38) |
EF-1a (0.50) |
UBQ (0.64) |
|
| 4 |
TUB (1.91) |
UBQ (1.83) |
CYP2 (1.17) |
18S (0.83) |
TUB (0.16) |
EF-1a (0.26) |
18S (0.37) |
F-box (0.26) |
UBQ (0.52) |
F-box (0.58) |
CYP2 (0.41) |
GAPDH (0.49) |
ACT1 (0.63) |
TUB (0.69) |
|
| 5 |
CYP2 (2.01) |
GAPDH (2.04) |
GAPDH (1.32) |
GAPDH (0.89) |
F-box (0.17) |
TUB (0.32) |
GAPDH (0.45) |
GAPDH (0.38) |
EF-1a (0.56) |
ACT1 (0.70) |
F-box (0.43) |
EF-1a (0.56) |
TUB (0.76) |
EF-1a (0.77) |
|
| 6 |
F-Box (2.03) |
TUB (2.2) |
TUB (1.45) |
TUB (0.95) |
ACT1 (0.25) |
CYP2 (0.45) |
CYP2 (0.59) |
18S (0.45) |
TUB (0.60) |
TUB (0.77) |
GAPDH (0.47) |
ACT1 (0.62) |
UBQ (0.82) |
18S (0.89) |
|
| 7 |
18S (2.19) |
EF-1a (2.41) |
F-box (1.69) |
ACT1 (1.03) |
18S (0.36) |
UBQ (0.58) |
TUB (0.75) |
EF-1a (0.55) |
CYP2 (0.68) |
EF-1a (0.9) |
ACT1 (0.57) |
18S (0.73) |
18S (0.96) |
CYP2 (1.02) |
|
| 8 |
EF-1a (2.29) |
18S (2.57) |
EF-1a (1.79) |
CYP2 (1.44) |
UBQ (0.52) |
GAPDH (0.71) |
EF-1a (0.86) |
ACT1 (0.76) |
GAPDH (0.76) |
GAPDH (1.07) |
UBQ (0.63) |
TUB (0.83) |
GAPDH (1.26) |
F-box (1.10) |
|
| Root | 1 |
ACT1 (1.11) |
F-box (1.08) |
18S (1.18) |
ACT1 (0.17) |
TUB (0.19) |
ACT1 (0.33) |
EF-1a (0.3) |
EF-1a (0.43) |
GAPDH (0.37) |
EF-1a (0.24) |
F-box (0.14) |
EF-1a (0.38) |
ACT1 (0.35) |
F-box (0.37) |
| 2 |
TUB (1.11) |
UBQ (1.08) |
UBQ (1.18) |
TUB (0.17) |
GAPDH (0.19) |
CYP2 (0.33) |
TUB (0.3) |
CYP2 (0.43) |
CYP2 (0.37) |
TUB (0.24) |
18S (0.14) |
GAPDH (0.38) |
CYP2 (0.35) |
ACT1 (0.37) |
|
| 3 |
F-box (1.65) |
ACT1 (1.54) |
ACT1 (1.31) |
F-box (0.24) |
F-box (0.21) |
TUB (0.39) |
18S (0.35) |
18S (0.51) |
UBQ (0.42) |
ACT1 (0.43) |
ACT1 (0.23) |
ACT1 (0.5) |
EF-1a (0.43) |
GAPDH (0.42) |
|
| 4 |
EF-1a (1.71) |
EF-1a (1.57) |
CYP2 (1.38) |
EF-1a (0.39) |
EF-1a (0.26) |
18S (0.42) |
UBQ (0.43) |
GAPDH (0.58) |
18S (0.58) |
UBQ (0.49) |
UBQ (0.38) |
18S (0.68) |
18S (0.54) |
TUB (0.50) |
|
| 5 |
GAPDH (1.78) |
TUB (1.69) |
TUB (1.43) |
CYP2 (0.57) |
CYP2 (0.27) |
UBQ (0.51) |
GAPDH (0.47) |
TUB (0.66) |
F-box (0.83) |
18S (0.56) |
TUB (0.46) |
TUB (0.85) |
UBQ (0.66) |
18S (0.71) |
|
| 6 |
18S (1.9) |
GAPDH (1.79) |
GAPDH (1.5) |
UBQ (0.63) |
ACT1 (0.33) |
F-box (0.61) |
F-box (0.58) |
ACT1 (0.71) |
EF-1a (0.94) |
F-box (0.64) |
CYP2 (0.5) |
F-box (0.97) |
GAPDH (0.79) |
EF-1a (0.77) |
|
| 7 |
UBQ (1.98) |
18S (1.91) |
F-box (1.76) |
GAPDH (0.72) |
UBQ (0.41) |
EF-1a (0.74) |
ACT1 (0.67) |
F-box (0.74) |
ACT1 (1.03) |
CYP2 (0.74) |
EF-1a (0.54) |
CYP2 (1.05) |
F-box (0.95) |
UBQ (0.80) |
|
| 8 |
CYP2 (2.08) |
CYP2 (2.19) |
EF-1a (1.9) |
18S (0.81) |
18S (0.49) |
GAPDH (0.86) |
CYP2 (0.89) |
UBQ (0.79) |
TUB (1.12) |
GAPDH (1.01) |
GAPDH (0.88) |
UBQ (1.11) |
TUB (1.14) |
CYP2 (1.48) |