Table 3.
Suitability ranking, based on the NormFinder program, of the eight candidate genes as potential reference genes for qPCR experiments.
| Tissue | Rank | Total | Abiotic | Hormone | P.vexans | SA | BTH | JA | ETH | GA3 | Heat | Cold | NA | Cd | PEG |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Leaf | 1 |
ACT1 (0.66) |
ACT1 (0.52) |
CYP2 (0.42) |
F-box (0.13) |
GAPDH (0.04) |
ACT1 (0.05) |
18S (0.13) |
UBQ (0.05) |
ACT1 (0.12) |
18S (0.04) |
CYP2 (0.07) |
CYP2 (0.07) |
TUB (0.24) |
EF-1a (0.18) |
| 2 |
UBQ (0.93) |
UBQ (0.59) |
GAPDH (0.43) |
UBQ (0.19) |
CYP2 (0.05) |
F-box (0.10) |
ACT1 (0.31) |
TUB (0.06) |
UBQ (0.18) |
CYP2 (0.04) |
F-box (0.17) |
UBQ (0.10) |
F-box (0.42) |
TUB (0.24) |
|
| 3 |
GAPDH (1.04) |
F-box (1.15) |
TUB (0.55) |
EF-1a (0.39) |
ACT1 (0.11) |
18S (0.17) |
UBQ (0.35) |
CYP2 (0.21) |
EF-1a (0.24) |
UBQ (0.37) |
18S (0.22) |
F-box (0.31) |
ACT1 (0.45) |
UBQ (0.40) |
|
| 4 |
F-box (1.12) |
GAPDH (1.18) |
ACT1 (0.78) |
18S (0.46) |
EF-1a (0.13) |
EF-1a (0.19) |
GAPDH (0.37) |
GAPDH (0.25) |
TUB (0.31) |
F-box (0.51) |
TUB (0.25) |
EF-1a (0.44) |
CYP2 (0.45) |
GAPDH (0.53) |
|
| 5 |
CYP2 (1.15) |
CYP2 (1.39) |
18S (1.10) |
TUB (0.54) |
TUB (0.18) |
TUB (0.22) |
CYP2 (0.39) |
F-box (0.27) |
F-box (0.37) |
TUB (0.56) |
EF-1a (0.30) |
ACT1 (0.45) |
UBQ (0.55) |
ACT1 (0.60) |
|
| 6 |
TUB (1.19) |
EF-1a (1.49) |
UBQ (1.17) |
GAPDH (0.63) |
F-box (0.24) |
CYP2 (0.42) |
F-box (0.43) |
18S (0.27) |
18S (0.44) |
EF-1a (0.61) |
ACT1 (0.46) |
GAPDH (0.49) |
18S (0.59) |
CYP2 (0.71) |
|
| 7 |
EF-1a (1.43) |
TUB (1.76) |
F-box (1.27) |
ACT1 (0.94) |
18S (0.44) |
UBQ (0.63) |
TUB (0.68) |
EF-1a (0.64) |
GAPDH (0.59) |
ACT1 (0.74) |
GAPDH (0.46) |
18S (0.53) |
EF-1a (0.66) |
18S (0.74) |
|
| 8 |
18S (1.46) |
18S (1.87) |
EF-1a (1.28) |
CYP2 (1.82) |
UBQ (0.67) |
GAPDH (0.72) |
EF-1a (0.74) |
ACT1 (0.93) |
CYP2 (0.61) |
GAPDH (1.05) |
UBQ (0.52) |
TUB (0.72) |
GAPDH (1.43) |
F-box (0.76) |
|
| Root | 1 |
ACT1 (0.38) |
ACT1 (0.22) |
TUB (0.41) |
ACT1 (0.07) |
TUB (0.13) |
CYP2 (0.03) |
18S (0.11) |
EF-1a (0.17) |
18S (0.18) |
TUB (0.08) |
EF-1a (0.02) |
ACT1 (0.24) |
ACT1 (0.12) |
ACT1 (0.13) |
| 2 |
TUB (0.75) |
EF-1a (0.65) |
CYP2 (0.52) |
TUB (0.11) |
GAPDH (0.13) |
18S (0.13) |
EF-1a (0.13) |
CYP2 (0.21) |
GAPDH (0.43) |
ACT1 (0.14) |
F-box (0.03) |
F-box (0.46) |
CYP2 (0.2) |
F-box (0.13) |
|
| 3 |
GAPDH (0.94) |
F-box (0.88) |
ACT1 (0.55) |
F-box (0.13) |
F-box (0.15) |
ACT1 (0.27) |
TUB (0.27) |
18S (0.33) |
F-box (0.48) |
EF-1a (0.15) |
ACT1 (0.07) |
EF-1a (0.54) |
EF-1a (0.26) |
GAPDH (0.15) |
|
| 4 |
UBQ (1.08) |
UBQ (0.93) |
GAPDH (0.66) |
EF-1a (0.39) |
ACT1 (0.17) |
F-box (0.34) |
UBQ (0.34) |
GAPDH (0.40) |
EF-1a (0.51) |
UBQ (0.20) |
UBQ (0.18) |
GAPDH (0.59) |
UBQ (0.41) |
TUB (0.18) |
|
| 5 |
F-box (1.09) |
TUB (0.99) |
UBQ (1.18) |
CYP2 (0.45) |
CYP2 (0.24) |
TUB (0.40) |
GAPDH (0.35) |
TUB (0.41) |
ACT1 (0.63) |
18S (0.36) |
18S (0.23) |
TUB (0.59) |
18S (0.52) |
18S (0.68) |
|
| 6 |
EF-1a (1.14) |
GAPDH (1.09) |
18S (1.20) |
UBQ (0.51) |
EF-1a (0.27) |
UBQ (0.55) |
ACT1 (0.50) |
ACT1 (0.42) |
CYP2 (0.71) |
F-box (0.53) |
TUB (0.47) |
CYP2 (0.62) |
GAPDH (0.57) |
EF-1a (0.71) |
|
| 7 |
CYP2 (1.32) |
18S (1.52) |
F-box (1.33) |
GAPDH (0.59) |
UBQ (0.33) |
EF-1a (0.63) |
F-box (0.53) |
F-box (0.55) |
UBQ (0.73) |
CYP2 (0.79) |
CYP2 (0.53) |
18S (0.65) |
F-box (0.95) |
UBQ (0.79) |
|
| 8 |
18S (1.32) |
CYP2 (1.94) |
EF-1a (1.42) |
18S (0.69) |
18S (0.48) |
GAPDH (0.77) |
CYP2 (1.04) |
UBQ (0.58) |
TUB (0.86) |
GAPDH (1.25) |
GAPDH (1.31) |
UBQ (0.78) |
TUB (1.12) |
CYP2 (2.41) |