Table 5.
Ranking the expression stabilities of eight candidate reference genes in ramie leaves and roots based on the RefFinder program.
| Tissue | Rank | Total | Abiotic | Hormone | P.vexans | SA | BTH | JA | ETH | GA3 | Heat | Cold | NA | Cd | PEG |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Leaf | 1 |
ACT1 (1.00) |
ACT1 (1.57) |
CYP2 (3.50) |
UBQ (1.19) |
GAPDH (1.5) |
ACT1 (1.41) |
ACT1 (1.41) |
UBQ (1.19) |
ACT1 (1.97) |
18S (1.19) |
TUB (1.86) |
CYP2 (1.41) |
box (1.41) |
EF-1a (1.50) |
| 2 |
UBQ (1.68) |
UBQ (2.00) |
18S (6.73) |
F-box (1.41) |
EF-1a (2.45) |
box (1.86) |
18S (1.86) |
TUB (1.57) |
UBQ (2.38) |
CYP2 (1.41) |
CYP2 (2.11) |
UBQ (1.86) |
CYP2 (1.68) |
TUB (2.38) |
|
| 3 |
GAPDH (3.83) |
F-box (2.59) |
ACT1 (1.57) |
18S (3.46) |
CYP2 (2.63) |
18S (2.06) |
UBQ (2.06) |
CYP2 (2.71) |
18S (2.45) |
UBQ (3.41) |
18S (2.63) |
F-box (3.41) |
TUB (2.94) |
GAPDH (3.25) |
|
| 4 |
F-box (4.43) |
CYP2 (3.50) |
GAPDH (4.86) |
EF-1a (3.83) |
TUB (2.99) |
EF-1a (3.72) |
F-box (4.36) |
F-box (4.23) |
F-box (2.94) |
F-box (4.43) |
F-box (3.31) |
18S (4.30) |
ACT1 (3.46) |
UBQ (3.57) |
|
| 5 |
CYP2 (5.00) |
GAPDH (4.86) |
TUB (6.96) |
TUB (4.95) |
F-box (4.16) |
TUB (5.00) |
GAPDH (4.47) |
GAPDH (4.73) |
EF-1a (4.05) |
TUB (4.95) |
EF-1a (3.87) |
EF-1a (4.86) |
EF-1a (4.53) |
ACT1 (3.76) |
|
| 6 |
TUB (5.63) |
EF-1a (5.24) |
UBQ (2.00) |
GAPDH (5.48) |
ACT1 (5.05) |
CYP2 (6.24) |
CYP2 (5.73) |
18S (6.24) |
TUB (5.09) |
EF-1a (5.24) |
GAPDH (4.74) |
GAPDH (5.42) |
UBQ (5.73) |
CYP2 (5.42) |
|
| 7 |
18S (5.86) |
18S (6.73) |
F-box (2.59) |
ACT1 (7.00) |
18S (7.00) |
UBQ (7.24) |
TUB (7.00) |
EF-1a (6.74) |
GAPDH (6.51) |
ACT1 (6.44) |
ACT1 (6.74) |
TUB (5.66) |
18S (6.74) |
F-box (5.86) |
|
| 8 |
EF-1a (7.20) |
TUB (6.96) |
EF-1a (2.54) |
CYP2 (8.00) |
UBQ (8.00) |
GAPDH (7.44) |
EF-1a (8.00) |
ACT1 (8.00) |
CYP2 (7.48) |
GAPDH (8.00) |
UBQ (8.00) |
ACT1 (5.89) |
GAPDH (8.00) |
18S (6.88) |
|
| Root | 1 |
ACT1 (1.19) |
ACT1 (1.73) |
TUB (2.34) |
TUB (1.41) |
TUB (1.57) |
CYP2 (1.41) |
EF-1a (1.19) |
EF-1a (1.19) |
18S (1.41) |
TUB (1.19) |
F-box (1.68) |
EF-1a (1.57) |
ACT1 (1.32) |
ACT1 (1.78) |
| 2 |
TUB (2.00) |
F-box (2.71) |
UBQ (2.45) |
ACT1 (1.57) |
F-box (1.73) |
18S (2.38) |
TUB (2.06) |
CYP2 (1.41) |
GAPDH (2.00) |
EF-1a (1.86) |
18S (2.11) |
ACT1 (1.57) |
CYP2 (2.00) |
F-box (1.93) |
|
| 3 |
GAPDH (3.41) |
EF-1a (2.83) |
18S (2.66) |
F-box (2.28) |
GAPDH (1.86) |
ACT1 (2.71) |
18S (2.21) |
18S (3.00) |
F-box (3.08) |
ACT1 (2.63) |
ACT1 (2.28) |
GAPDH (2.63) |
EF-1a (2.71) |
TUB (3.72) |
|
| 4 |
18S (4.14) |
UBQ (3.25) |
CYP2 (2.91) |
EF-1a (4.23) |
CYP2 (4.73) |
F-box (3.31) |
UBQ (3.72) |
GAPDH (4.23) |
CYP2 (3.83) |
UBQ (3.94) |
EF-1a (3.44) |
F-box (3.83) |
18S (3.16) |
EF-1a (3.83) |
|
| 5 |
F-box (4.68) |
18S (4.30) |
ACT1 (3.08) |
CYP2 (5.23) |
EF-1a (4.90) |
TUB (4.16) |
GAPDH (5.00) |
ACT1 (5.18) |
EF-1a (4.12) |
18S (4.40) |
UBQ (3.94) |
TUB (5.23) |
UBQ (4.68) |
GAPDH (3.83) |
|
| 6 |
UBQ (5.14) |
GAPDH (4.56) |
GAPDH (4.43) |
UBQ (5.42) |
ACT1 (5.18) |
EF-1a (5.66) |
F-box (6.24) |
TUB (5.48) |
ACT1 (5.44) |
F-box (6.00) |
TUB (5.73) |
18S (5.29) |
GAPDH (6.24) |
18S (3.98) |
|
| 7 |
EF-1a (5.42) |
TUB (5.00) |
F-box (7.24) |
GAPDH (7.00) |
UBQ (7.00) |
UBQ (6.16) |
ACT1 (6.74) |
F-box (7.24) |
UBQ (5.66) |
CYP2 (7.24) |
CYP2 (6.74) |
CYP2 (6.19) |
F-box (6.44) |
UBQ (5.66) |
|
| 8 |
CYP2 (7.74) |
CYP2 (8.00) |
EF-1a (7.74) |
18S (8.00) |
18S (8.00) |
GAPDH (7.74) |
CYP2 (8.00) |
UBQ (7.74) |
TUB (8.00) |
GAPDH (7.74) |
GAPDH (8.00) |
UBQ (8.00) |
TUB (8.00) |
CYP2 (8.00) |