Figure 1.

Experimental workflow for MDR screening of cancer cells using MCSs. At the starting point, PTPD tumor biopsies were collected from the gingiva, tongue, and hypopharynx. During the intermediate point, cell lines were established from the pretreated biopsy specimens and in vitro MCSs cultures were generated using these cell lines. In the endpoint, classification of cancer cells as MDR or non-MDR was performed by combining drug screening cell cytotoxicity assay, real-time monitoring of drug uptake and ROS, and FACs based confirmation of MDR profile.