Table 3.
mRNA expression of BDNF, TrkB, FNDC5, AT1R, and MR in brain areas of SHR and WKY with or without exercise training
| WKY | SHR | |||
|---|---|---|---|---|
| Sed (n = 10) | ExT (n = 7) | Sed (n = 6) | ExT (n = 9) | |
| BDNF | ||||
| PVN mRNA/PGK1 (×10–2) | 4.3 ± 0.5 | 3.7 ± 0.3 | 4.0 ± 0.4 | 4.2 ± 0.3 |
| RVLM mRNA/PGK1 (×10–2) | 1.6 ± 0.2 | 1.7 ± 0.4 | 2.4 ± 0.5 | 2.0 ± 1.2 |
| CA mRNA/PGK1(×10–2) | 11.1 ± 1 | 11.3 ± 0.8 | 9.8 ± 2 | 10.3 ± 2 |
| DG mRNA/PGK1(×10–2) | 11.5 ± 1.0 | 10.2 ± 0.8 | 7.8 ± 1.3* | 10.1 ± 2.4*# |
| TrkB | ||||
| PVNmRNA/PGK1 (×10–1) | 3.1 ± 0.08 | 3.4 ± 0.3 | 3.8 ± 0.4* | 3.9 ± 0.06* |
| RVLM mRNA/PGK1 (×10–1) | 1.6 ± 0.2 | 1.6 ± 0.2 | 1.6 ± 0.1 | 1.6 ± 0.2 |
| CA mRNA/PGK1(×10–1) | 2.2 ± 0.3 | 2.3 ± 0.5 | 2.9 ± 0.3* | 2.9 ± 0.8* |
| DG mRNA/PGK1(×10–1) | 3.3 ± 0.3 | 2.7 ± 0.8 | 3.6 ± 0.3* | 3.7 ± 0.3* |
| FNDC5 | ||||
| PVN mRNA/PGK1 (×10–1) | 1.7 ± 0.3 | 1.6 ± 0.1 | 1.7 ± 0.3 | 1.7 ± 0.08 |
| RVLM mRNA/PGK1 (×10–1) | 3.0 ± 0.4 | 2.8 ± 0.3 | 2.8 ± 0.6 | 2.8 ± 0.4 |
| CA mRNA/PGK1(×10–1) | 3.4 ± 0.1 | 3.1 ± 0.6 | 3.2 ± 0.1 | 3.1 ± 0.5 |
| DG mRNA/PGK1(×10–1) | 2.6 ± 0.2 | 2.3 ± 0.5 | 2.5 ± 0.2 | 2.4 ± 0.2 |
| AT1R | ||||
| PVN mRNA/PGK1 (×10–2) | 3.2 ± 0.5 | 3.4 ± 0.8 | 3.5 ± 0.2 | 4.1 ± 0.5 |
| RVLM mRNA/PGK1 (×10–2) | 1.7 ± 0.1 | 1.5 ± 0.4 | 1.4 ± 0.3 | 1.4 ± 0.3 |
| CA mRNA/PGK1(×10–2) | 0.7 ± 0.4 | 0.7 ± 0.4 | 0.5 ± 0.2 | 0.6 ± 0.3 |
| DG mRNA/PGK1(×10–2) | 1.7 ± 0.2 | 1.4 ± 0.3 | 1.1 ± 0.1* | 1.1 ± 0.4* |
| MR | ||||
| PVN mRNA/PGK1 (×10–2) | 1.7 ± 0.3 | 1.4 ± 0.2 | 1.5 ± 0.2 | 1.5 ± 0.1 |
| RVLM mRNA/PGK1 (×10–2) | 2.2 ± 0.2 | 1.7 ± 0.4 | 1.4 ± 0.3* | 1.3 ± 0.2* |
| CA mRNA/PGK1(×10–2) | 11.9 ± 2.3 | 13.1 ± 3.3 | 12.9 ± 2.5 | 13.4 ± 3.1 |
| DG mRNA/PGK1(×10–2) | 12.7 ± 0.2 | 9.5 ± 0.3§ | 9.9 ± 0.1§ | 12.6 ± 0.2 |
Values are means ± SD (n = 4/group). For BDNF in the PVN: SHR versus WKY, F = .12, p = .73 NS; ExT versus Sed, F = .65, p = .44 NS; ExT × strain interaction, F = 3.99, p = .07 NS. For BDNF in the RVLM: SHR versus WKY, F = 2.40, p = .15 NS; ExT versus Sed, F = .38, p = .55 NS; ExT × strain interaction, F = .53, p = .48 NS. For BDNF in the CA: SHR versus WKY, F = 2.18, p = .17 NS; ExT versus Sed, F = .22, p = .65 NS; ExT × strain interaction, F = .01, p = .92 NS. For BDNF in the DG: SHR versus WKY, F = 10.77, p = .0066; ExT versus Sed, F = .93, p = .35 NS; ExT × strain interaction, F = 6.75, p = .023. For TrkB in the PVN: SHR versus WKY, F = 22.27, p = .0005. ExT versus Sed, F = 3.08, p = .11 NS; ExT × strain interaction, F = .29, p = .60 NS. For TrkB in the RVLM: SHR versus WKY, F = .05, p = .83 NS; ExT versus Sed, F = .001, p = .97 NS; ExT × strain interaction, F = .07, p = .80 NS. For TrkB in the CA: SHR versus WKY, F = 5.90, p = .032; ExT versus Sed, F = .12, p = .74 NS; ExT × strain interaction, F = .06, p = .81 NS. For TrkB in the DG: SHR versus WKY, F = 7.02, p = .021; ExT versus Sed, F = .97, p = .34 NS; ExT × strain interaction, F = 2.44, p = .14 NS. For FNDC5 in the PVN: SHR versus WKY, F = .22, p = .65 NS; ExT versus Sed, F = .10, p = .76 NS; ExT × strain interaction, F = .16, p = .69 NS. For FNDC5 in the RVLM: SHR versus WKY, F = .31, p = .59 NS; ExT versus Sed, F = .36, p = .56 NS; ExT × strain interaction, F = .09, p = .77 NS. For FNDC5 in the CA: SHR versus WKY, F = .13, p = .73 NS; ExT versus Sed, F = 1.22, p = .29 NS; ExT × strain interaction, F = .21, p = .66 NS. For FNDC5 in the DG: SHR versus WKY, F = .004, p = .95 NS; ExT versus Sed, F = 1.53, p = .24 NS; ExT × strain interaction, F = .38, p = .55 NS. For AT1R in the PVN: SHR versus WKY, F = 3.40, p = .09 NS; ExT versus Sed, F = 1.60, p = .23 NS; ExT × strain interaction, F = .61, p = .45 NS. For AT1R in the RVLM: SHR versus WKY, F = 2.14, p = .17 NS; ExT versus Sed, F = .49, p = .50 NS; ExT × strain interaction, F = .25, p = .62 NS. For AT1R in the CA: SHR versus WKY, F = .97, p = .35 NS; ExT versus Sed, F = .06, p = .81 NS; ExT × strain interaction, F = .35, p = .57 NS. For AT1R in the DG: SHR versus WKY, F = 9.77, p = .0096; ExT versus Sed, F = .59, p = .46 NS; ExT × strain interaction, F = .87, p = .37 NS. For MR in the PVN: SHR versus WKY, F = .07, p = .79 NS; ExT versus Sed, F = 2.76, p = .12 NS; ExT × strain interaction, F = 2.12, p = .17 NS. For MR in the RVLM: SHR versus WKY, F = 15.68, p = .002; ExT versus Sed, F = 4.62, p = .05 NS; ExT × strain interaction, F = 2.56, p = .14 NS. For MR in the CA: SHR versus WKY, F = .20, p = .66 NS; ExT versus Sed, F = .38, p = .55 NS; ExT × strain interaction, F = .08, p = .78 NS. For MR in the DG: SHR versus WKY, F = .03, p = .87 NS; ExT versus Sed, F = .02, p = .90 NS; ExT × strain interaction, F = 6.85, p = .0225.
p < .05 versus WKY.
p < .05 versus SHR‐Sed.
p < .05 versus WKY‐Sed or SHR‐ExT.