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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Magn Reson. 2019 Nov 11;310:106646. doi: 10.1016/j.jmr.2019.106646

Fig. 1.

Fig. 1.

(A) Schematic representations of NPM1 and p14ARF, where the NPM1 acidic and basic tracts are denoted as A1–3 and B1–2, respectively, and p14ARF arginine motifs are denoted as R1–3. (B, top) Standard curves for unconjugated Alexa Fluor 488 and Alexa Fluor 405. Error bars representing the standard deviation are smaller than the markers. (B, bottom) Concentrations of NPM1 and p14ARF in non-centrifuged droplets and droplets that were ultracentrifuged at 100,000 rpm (436,000 xg). Error bars represent the propagation of error from 4 replicates (C, top) FRAP of Alexa Fluor 488 labeled NPM1 and Alexa Fluor 405 labeled p14ARF within individual droplets, where the scale bar = 2 μm. (C, bottom) FRAP recovery curves for Alexa Fluor 488 labeled NPM1 and Alexa Fluor 405 labeled p14ARF, where the error bars represent the standard deviation of n=12 droplets each. (D) Average fluorescence intensity of Alexa Fluor 488 labeled NPM1 within the NPM1-p14ARF condensed phase plotted as a function of temperature. Error bars representing the standard deviation are smaller than the markers. Representative images are shown above, where the scale bar = 20 μm. (E) Image of a 50 ml suspension of [U13C, U15N]-NPM1-p14ARF droplets collected immediately after phase separation. (F) The [U13C, U15N]-NPM1-p14ARF condensed phase after centrifugal sedimentation.