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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Comp Biochem Physiol B Biochem Mol Biol. 2019 Oct 24;240:110368. doi: 10.1016/j.cbpb.2019.110368

Figure 3. Generation of Tg(ef1α:MuRF1a-GFP) and Tg(ef1α:MuRF2a-GFP) transgenic zebrafish, Danio rerio.

Figure 3.

A: Diagram of the Tg(ef1α:MuRF1a-GFP) and Tg(ef1α:MuRF2a-GFP) transgenes. The full-length coding sequences of zebrafish murf1a or murf2a were cloned in frame upstream of the EGFP coding sequence in the pT2AL200R150G vector. The pT2AL200R150G vector contained an elongation factor-1 alpha (EF-1α) gene promoter. Transgenic zebrafish were generated using these two transgenes.

B-D: Subcellular localization of MuRF1a-GFP (B), MuRF1a-GFP (D), or XBP1-GFP fusion protein in myofibers of the respective transgenic zebrafish embryos at 28 hpf. Scale bar: 30 μm.