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DNA plasmids for antisense RNA probes
| DNA plasmids | RNA polymerase | Restriction Enzymes |
|---|---|---|
| Murf1a | T7 | NarI |
| Murf1b | SP6 | HindIII |
| Murf2a | T7 | StuI |
| Murf2b | SP6 | NarI |
| Murf3 | SP6 | EcoRV |
| Murf2-like | SP6 | StuI |
The DNA plasmids used to generate the anti-sense probes for in situ hybridization in zebrafish (Danio rerio) embryos. Each plasmid was digested with a specific restriction enzyme and used as template for RNA synthesis using respective T7 or Sp6 RNA polymerase.
