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. 2019 Summer;18(3):1580–1594. doi: 10.22037/ijpr.2019.1100762

Table 4.

Primer set sequences for amplification of 5 virulence factors encoding genes

Primer names Primer sequences from 5' to 3' direction Name of target gene PCR product length
ExoS Forward CGTATGAGTCAGCAAGGGCG
ExoS 118 bp
ExoS Reverse GCGATGTGGTCACTGGCTTC
ETA Forward GACAACGCCCTCAGCATCACCAGC
ETA 396 bp
ETA Reverse CGCTGGCCCATTCGCTCCAGCGCT
oprL Forward ATGGAAATGCTGAAATTCGGC oprL
504 bp
oprL Reverse CTTCTTCAGCTCGACGCGACG oprL
oprI Forward ATGAACAACGTTCTGAAATTCTCTGCT oprI
249 bp
oprI Reverse CTTGCGGCTGGCTTTTTCCAG oprI
Alg Forward TTCCCTCGCAGAGAAAACATC Alg
520 bp
Alg Reverse CCTGGTTGATCAGGTCGATCT Alg