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. 2019 Nov 1;31(1):67–83. doi: 10.1681/ASN.2019020125

Figure 5.

Figure 5.

LS transgenic zebrafish have selectively shortened megalin/lrp2a-positive pronephric segments. In situ hybridization of megalin/lrp2a (PT marker) (A and D), cdh17 (pronephric tubule marker) (B and E), and clcnk (distal tubule marker) (C and F) was performed in WT (ocrl+/+), heterozygous mutant (ocrl+/−), and homozygous mutant (ocrl−/−) transgenic zebrafish at 72 hpf. The distributions of lrp2a (A), cdh17 (B), and clcnk (B), and expression in representative zebrafish, are shown. Scatter plots show the lengths of lrp2a- (D), cdh17- (E), and clcnk- (E) positive segments measured in ocrl+/+ and ocrl+/− zebrafish compared with ocrl−/− zebrafish (mean±SD; lrp2a, n=40–92; cdh17, n=33–56; clcnk, n=17–47). Ratios of average lrp2a (G) and clcnk (H) lengths over average cdh17 length were compared among ocrl+/+, ocrl+/−, and ocrl−/− zebrafish. ****P<0.001 on the basis of an unpaired t test in (B).