Figure 1.

IL-37 overexpression did not affect the stem cell characteristics of MSCs. MSCs were isolated from healthy MRL/MpJ mice. They were infected with the lentivirus expressing IL-37 (MSCs-IL37) or negative control (MSCs-Flag) at passage 3. (A) Cell morphology of the mice MSCs-IL37 or MSCs-Flag at passage 6. (B) Western blot showed the expression of IL-37 in the MSCs-IL37 at passage 6. (C) Flow cytometry analysis of the mice MSCs-IL37 at passage 6. (D) Differentiation of adipocytes, osteocytes, and chondrocytes of MSCs-IL37 and MSCs at passage 6. Scale bar, 200 μm. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.