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. 2019 Sep 19;31(1):23–38. doi: 10.1681/ASN.2019040337

Figure 1.

Figure 1.

Successful translational profiling of PT using the SLC34a1-eGFPL10a line. (A) Schematic illustrating the experimental work flow. (B) Trichrome stain showing collagen deposition in the kidney interstitium (male mice) after day 5 (D5UUO) and 10 (D10UUO) UUO. (C) Perinuclear and nucleolar expression of eGFP in PT (male mice), a pattern consistent with ribosomal location. Scale bar, 50 μm. (D) Immunofluorescence staining shows a strong induction of interstitial αSMA in the UUO kidney (male mice) and no expression in eGFP-positive proximal tubular cells. (E) Sample clustering in MDS plot. Scale bar, 50 μm. (F) Robust enrichment of PT-specific genes through scatterplot of normalized expression values in bound RNA (PT) versus unbound RNA (whole cortex). (G) Validation of cell type specificity of the SLC34a1-eGFPL10a line using quantitative PCR. PT markers, Slc34a1, Slc5a2 (Sglt2) and Slc2a2 (Glut2); podocyte markers, Nphs1 and Synpo; loop of Henle markers, Slc12a1 and Umod; collecting duct markers, Aqp2 and Aqp4; endothelial markers, Emcn and Pecam1; myofibroblast markers, Col1a1 and Acta2. Statistical analysis was performed using one-way ANOVA to compare data among groups (four male mice per group). Ab, antibody; CPM, count per million; DAPI, 4′,6-diamidino-2-phenylindole.