Figure 3.

Validation of PT-specific injury signature in fibrosis. (A) Volcano plots revealing widespread gene expression changes in PT beginning at UUO day 5 (D5UUO). Longer kidney obstruction time (day 10 [D10UUO]) altered the PT transcriptome only minimally. (B) RNA ISH of select PT injury responsive genes identified from TRAP analysis. Representative images were obtained from female kidneys. Red boxes indicate region enlarged. (C) Heatmap illustrating genes whose expression are altered in fibrotic PT but not detected in whole cortex. (D) Heatmap of expression profiles in all samples for DEGs that are changed during UUO in whole cortex but not in the PT. FDR<0.05, log fold change >1 or less than −1.