Figure 4.

Evaluation of epithelial-to-mesenchymal transition, fatty acid metabolism, and hedgehog ligand gene expression signatures. (A) Heatmap showing the expression of EMT markers in whole kidney but not PT. (B) Gene set enrichment analysis (GSEA) confirms defective fatty acid metabolism in PT during UUO. The total height of the curve indicates the extent of enrichment, with the normalized enrichment score (ES), P value, and FDR value indicated. (C) Heatmap of leading edge genes in the fatty acid metabolism gene set. (D) Expression of Ihh in PT by reads per kilobase of transcript per million mapped reads (RPKM) value. (E) Expression of Shh in PT by RPKM value. D5UUO, 5 days after UUO; D10UUO, 10 days after UUO.