Figure 6.

Many lncRNAs are detected and undergo differential regulation in PT during fibrosis. (A) MA plot displaying the differentially expressed lncRNAs in PT (UUO versus CLK). (B) MDS plot for the PT TRAP samples according to lncRNA expression profiles alone. (C) ISH validates two PT lncRNAs selected from the DEG list (UUO versus CLK). Data were obtained from male kidneys. Red boxes indicate region enlarged. (D) Network approach to show the protein coding mRNAs that might be targeted by Snhg18 and Gm20513. Nodes are lncRNAs (circle) and their target mRNAs (square). Edge sizes are determined by the energy (absolute log scale) required for lncRNA-mRNA interaction (LncRRI tools). Heatmap showing the target genes that are differentially expressed in PT during UUO. D5UUO, 5 days after UUO; D10UUO, 10 days after UUO; F, female; log2FC, log₂ fold change; M, male; RPKM; reads per kilobase of transcript per million mapped reads.