Periostin interacts with Itgβ1 on epithelial cells after I/R. (A) Itgβ1 is gradually upregulated in TECs after I/R, (B) whereas its levels are similarly induced in control and periostin-overexpressing mice, or (C) WT and periostin KO mice. (D) Coimmunoprecipitation with an Itgβ1 antibody showed that Itgβ1 interacts with periostin after I/R. Representative images are shown. Scale bars, 50 μm. *P<0.05, **P<0.01, ***P<0.001 versus CNT, POSTN, WT, or KO; §P<0.05, §§P<0.01 versus I/R 24 hours. n=4 for CNT or CTL and 6 per I/R group. CTL, control contralateral kidney; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.