KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| Urea | AMRESCO | Cat# 0378–5KG |
| TRIS | AMRESCO | Cat# 0497–5KG |
| Toluidine Blue | AMRESCO | Cat# 0672–25G |
| HEPES | Sigma-Aldrich | Cat# H3375–250G |
| Sodium citrate tribasic dihydrate | Sigma-Aldrich | Cat# C8532–1KG |
| PEG 20,000 | Sigma-Aldrich | Cat# 81300–1KG |
| Glycerol | Sigma-Aldrich | Cat# G5516–1L |
| T7 RNA polymerase | Home-made | N/A |
| Spermindine | Sigma-Aldrich | Cat# S2626–1G |
| [α-32P] ATP | PerkinElmer | Cat# NEG503H250UC |
| ATP | Sigma-Aldrich | Cat# A2383 |
| UTP | Sigma-Aldrich | Cat# U6625 |
| CTP | Sigma-Aldrich | Cat# C1506 |
| GTP | Sigma-Aldrich | Cat# G8877 |
| AMPPNP | Sigma-Aldrich | Cat# A2647–25MG |
| AxyPrep Plasmid Maxiprep Kit 25-prep | Axygen | Cat# AP-MX-P-25G |
| Critical Commercial Assays | ||
| Chelating Sepharose Fast Flow | GE Healthcare | Cat# 17-0575-02 |
| HiTrap Heparin | GE Healthcare | Cat# 17-1152-01 |
| Superdex 200 Increase, 10/300 GL | GE Healthcare | Cat# 28-9909-44 |
| Deposited Data | ||
| The coordinates of SthCsm binary complex | This study | PDB: 6IFN |
| The coordinates of SthCsm-NTR complex | This study | PDB: 6IFL |
| The coordinates of SthCsm-CTR1 complex | This study | PDB: 6IFY |
| The coordinates of SthCsm-CTR2-bubbled DNA complex | This study | PDB: 6IFU |
| The coordinates of SthCsm-CTR2-ssDNA complex | This study | PDB: 6IFZ |
| The coordinates of SthCsm-CTR1-AMPPNP complex | This study | PDB: 6IFK |
| The coordinates of SthCsm-CTR1-ATP complex | This study | PDB: 6IG0 |
| The coordinates of SthCsm-NTR-ATP complex | This study | PDB: 6IFR |
| The density map of SthCsm-NTR complex | This study | EMDB: EMD-9654 |
| The density map of SthCsm-CTR1 complex | This study | EMDB: EMD-9658 |
| The density map of SthCsm-CTR2-bubbled DNA complex | This study | EMDB: EMD-9657 |
| The density map of SthCsm-CTR2-ssDNA complex | This study | EMDB: EMD-9659 |
| The density map of SthCsm-CTR1-AMPPNP complex | This study | EMDB: EMD-9653 |
| The density map of SthCsm-CTR1-ATP complex | This study | EMDB: EMD-9660 |
| The density map of SthCsm-NTR-ATP complex | This study | EMDB: EMD-9656 |
| Experimental Models: Organisms/Strains | ||
| Escherichia coli BL21 (DE3) | Novagen | Cat# 69450 |
| Thermus thermophilus HB27 | Henne et al., 2004 | N/A |
| Thermus thermophilus HB27 III-A+ | This study | N/A |
| Thermus thermophilus HB27 III-A− | This study | N/A |
| Recombinant DNA | ||
| pCDFDuet-Csm1-Csm4, various mutants | This study | N/A |
| pRSFDuet-His6-Csm3-Csm2, various mutants | This study | N/A |
| pETDuet-Csm5 | This study | N/A |
| pACYCDuet-Cas6-pre-crRNA | This study | N/A |
| Oligonucleotides | ||
| RNA and DNA | This study | Table S4 |
| Software and Algorithms | ||
| PHENIX | Adams et al., 2002 | http://www.phenix-online.org/ |
| Coot | Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PyMOL | Molecular Graphics System, Version 1.8 Schrodinger | https://pymol.org/2/ |
| MotionCor2 | Zheng et al., 2017 | http://msg.ucsf.edu/em/software/motioncor2.html |
| CTFFind4 | Rohou and Grigorieff, 2015 | http://grigoriefflab.janelia.org/ctffind4 |
| RELION2.0 | Kimanius et al., 2016 | http://www2.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page |
| USCF Chimera | Pettersen et al., 2004 | https://www.cgl.ucsf.edu/chimera/ |
| ResMap | Kucukelbir et al., 2014 | http://resmap.sourceforge.net/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| Amicon Ultra Centrifugal Filters Ultracel-30K | Merck Millipore Ltd | Cat# UFC903096 |
| 3 Well Midi Crystallization Plate (Swissci) | HAMPTON RESEARCH | Cat# HR3–125 |
| GiG-C322 grids (2 mm holes and spacing) | Beijing Zhongjingkeyi Technology | Cat# GIG-2020–300 |