Figure 5. Phenotype of the startle response of Trh and serotonin receptor mutants.

A. Dotplot showing median population walking speed during a five-minute recording session for Trh⁰¹ mutants (blue), which walk faster than background matched isoCS controls (black), consistent with the 5-HT^VNC inactivation experiments. 5-HT7^Gal4 mutants (purple) also walk faster than controls, but the other receptor mutants do not. ***p<.001, **p<.01, *p<.05 ns p>.05 by Kruskal-wallis analysis with Dunn-sidak correction for multiple comparisons. Brown box indicates median. N= isoCS (130) 5-HT1A^Gal4 (130) 5-HT1B^Gal4 (120) 5-HT2A^Gal4 (100) 5-HT7^Gal4 (120) Trh⁰¹ (120).

B-F. Median population walking speed sampled at 30 Hz in response to vibration stimulus. Trh⁰¹ mutants (B, blue line) show a blunted and shortened pause in response to the novel stimulus. 5-HT7^Gal4 mutants (C, purple line) and 5-HT1B^Gal4 mutants (D, red line) show a similar phenotype to Trh⁰¹ mutants. 5-HT2A^Gal4 mutants (E, green line) and 5-HT1A^Gal4 mutants (F, yellow line) have a pause phase comparable to controls, but do not accelerate as much in response to the vibration stimulus. N= isoCS (140) 5-HT1A^Gal4 (115) 5-HT1B^Gal4 (124) 5-HT2A^Gal4 (88) 5-HT7^Gal4 (120) Trh⁰¹ (139)

G. Heatmap showing how lack of Trh or serotonergic receptors affects the response to vibration stimulus. Plotted for every genotype is the difference in mean ranks (Kruskal-wallis test with Dunn-sidak correction for multiple comparisons statistics) for each parameter compared to isoCS control flies. Starred parameters are those where differences between control and experimentals consistently reached significance (p<.05, see STAR Methods).

See also Figure S5.