| Antibodies |
|
| Rabbit anti Vglut |
Aaron DiAntonio [94] |
|
1:10,000 |
| Rabbit anti 5-HT |
Millipore Sigma |
RRID:AB_477522 |
1:1000 |
| Mouse anti TH |
Immunostar |
RRID:AB_572268 |
1:1000 |
| Mouse anti ChAT4B1 |
Developmental Studies Hybridoma Bank (DSHB) |
RRID:AB_528122 |
1:500 |
| Mouse anti nc82 c |
DHSB |
RRID:AB_2314866 |
1:20 – 1:100 |
| Rabbit anti GABA |
Millipore Sigma |
RRID:AB_477652 |
1:1000 |
| Rabbit anti Tdc2 |
Cova Labs |
pab0822-P |
1:200 |
| Rabbit anti dsRed |
Takara Bio |
RRID:AB_10013483 |
1:1000 |
| Experimental Models: Strains of D. melanogaster |
|
| ; ; Trh Gal4 |
Bloomington Drosophila Stock Center (BDSC) [48] |
RRID:BDSC_38389 |
|
| ; ; TH Gal4 |
BDSC [55] |
RRID:BDSC_8848 |
|
| ; Tdc2 Gal4 ; |
BDSC [56] |
RRID:BDSC_9313 |
|
| w1118 ; IIiso ; IIIiso |
BDSC [98] |
RRID:BDSC_5905 |
|
| w1118 ; IIiso ; Trh-Gal4 |
This study |
|
|
| w1118 ; IIiso ; TH-Gal4 |
This study |
|
|
| w1118 ; Tdc2 Gal4 ; IIIiso |
This study |
|
|
| w1118 ; IIiso ; Trh iso Gal4 |
This study |
|
outcrossed 10 x to w1118 ; IIiso ; IIIiso
|
| ; UAS-mCD8::GFP; |
[99] |
|
|
| tub>gal80> ; tsh-LexA, LexAop-Flp ; |
[100] |
|
Tsh LexA made by Julie Simpson. Combined tool provided by Wes Grueber |
| ; tsh-Gal80 ; |
Julie Simpson |
|
|
| ; ; UAS-csChrimson::mVenus |
BDSC [101] |
RRID:BDSC_55136 |
|
| ; ; UAS-Kir2.1 |
BDSC [102] |
RRID:BDSC_6595 |
|
| ; ; 20XUAS-hexameric-GFP |
BDSC / Steve Stowers [103] |
RRID:BDSC_52262 |
|
| ; UAS-OpGCamp6f ; UAS-tdTomato |
Pavan Ramdya [59] |
|
|
| IsoCS |
Yi Rao [47] |
|
|
| w+ ; 5-HT1AGal4 ; |
Yi Rao [47] |
|
|
| w+ ; 5-HT1BGal4 ; |
Yi Rao [47] |
|
|
| w+; ; 5-HT2AGal4 |
Yi Rao [47] |
|
|
| w+; ; 5-HT7Gal4 |
Yi Rao [47] |
|
|
| w+; ; Trh01 |
Yi Rao [47] |
|
|
| ; 5-HT1A-Gal4 (MI04464); |
Herman A. Dierick [75] |
|
|
| ; 5-HT1B-Gal4 (MI05213); |
Herman A. Dierick [75] |
|
|
| ; ; 5-HT2A-Gal4 (MI00459) |
Herman A. Dierick [75] |
|
|
| ; ; 5-HT2A-Gal4 (MI03299) |
Herman A. Dierick [75] |
|
|
| ; ; 5-HT2B-Gal4 (MI05208) |
Herman A. Dierick [75] |
|
|
| ; ; 5-HT2B-Gal4 (MI06500) |
Herman A. Dierick [75] |
|
|
| ; ; 5-HT2B-Gal4 (MI07403) |
Herman A. Dierick [75] |
|
|
| ; ; 5-HT7-Gal4 (MI00215) |
Herman A. Dierick [75] |
|
|
| Vglut>>LexAVP16, LexO-CD8GFP /FM7; Vglut>>LexAVP16, UASFlp, LexO-CD8GFP /CyO; LexO-CD8GFP, LexO-CD8GFP, Vglut>>LexAVP16 /TM2 |
Myungin Baek |
|
|
| ; Mhc-RFP ; |
BDSC |
RRID:BDSC_38464 |
|
| Software and Algorithms |
|
|
|
| Fiji |
[95] |
https://fiji.sc |
|
| Caltech FlyTracker |
[58] |
http://www.vision.caltech.edu/Tools/FlyTracker/index.html |
|
| Flywalker |
[27] |
|
|
| Flycapture |
|
https://www.flir.com/products/flycapture-sdk |
|
| Fview2 |
Andrew Straw, modified from [97] |
|
|
| MATLAB 2018a |
Mathworks |
https://www.mathworks.com/products/matlab.html |
|
| R Studio 3.3.2 |
|
https://www.rstudio.com |
|
| Python 2.7 Anaconda |
|
https://www.anaconda.com/distribution/ |
|
| NIS Elements AR |
Nikon |
https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
|
| PuTTY |
|
https://www.chiark.greenend.org.uk/~sgtatham/putty/ |
|
| Arduino IDE 1.8.5 |
Arduino |
https://www.arduino.cc/en/Main/Software |
|
