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. 2019 Dec 27;19:1252. doi: 10.1186/s12885-019-6474-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of AGGF1 in CRC tissues and paired normal mucosa. a. Western blot analysis was used to detect AGGF1 protein expression in eight representative paired CRC tissue samples, with GAPDH used as the loading control. b–i: Immunohistochemical staining showed that AGGF1 protein expression was significantly higher in CRC tissues compared with adjacent normal mucosa, with AGGF1 staining mainly observed in the cytoplasm of CRC cells. b, f: Negative AGGF1 staining in normal colorectal epithelium; c, g: Weak AGGF1 staining in well-differentiated CRC; d, h: Intense AGGF1 staining in moderately differentiated CRC; e, i: Strong AGGF1 staining in poorly differentiated CRC; b–e. Original magnification: 50×; f–i. Original magnification: 400 ×