Fig. 6.

Loss of HIF signaling does not impair hypoxia-induced glycolysis. a Growth curves of K562 HIF1, HIF2, and ARNT knockout cells under hypoxia and normoxia. b–c Glucose consumption (b) and lactate production (c) of K562 HIF1, HIF2, and ARNT knockout cells grown under normoxic and hypoxic growth conditions for 24 h. *p < 0.05. d 1D 1H-NMR extract metabolite intensities from K562 and ARNT knockout cells grown under hypoxia or normoxia for 24 h. e 1D 1H-NMR medium metabolite intensities from K562 wildtype (wt) and ARNT knockout cells grown under hypoxia and normoxia with medium collection at 18, 21, and 24 h for analysis to calculate the rate of production/consumption of indicated metabolites