Fig. 1.

Decrease in the biliary cholesterol secretion in L1-Tg mice. a Immunoblot detection of hepatic NPC1L1 protein in L1-Tg mice using an anti-NPC1L1 antibody. WT, wild-type. b Lower rates of biliary cholesterol secretion in L1-Tg mice fed a high-fat diet (HFD) for 2 weeks than those in WT mice. c Bile flow. d Biliary secretion of bile acids. e Biliary secretion of phospholipids. Data are expressed as the mean ± SEM. n = 4 (WT), 6 (L1-Tg). Statistical analyses for significant differences were performed using a two-sided t-test (††, P < 0.01; NS, not significantly different between groups)