Fig. 3.

Coverage of duplexed oligos containing single-stranded overhangs for SRSLY and NEBNext. (a) Cartoon schematic of duplexed synthetic oligos – one blunt end, an identifiable 50 nt complementary region, and an overhang of specific length and type. (b) Average coverage per base across the length of all duplexed oligos for three technical replicates in 0 base coordinates for both SRSLY and NEBNext methods. Technical replicates were not statistically different from each other (Students t-test: SRSLY p = 0.714, NEBNext p = 0.985), error bars not shown for aesthetics. Each oligo sequenced > 5000 reads