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. 2019 Dec 24;10(67):7122–7131. doi: 10.18632/oncotarget.27390

Figure 1. Induction of ISG15 expression by L1 in CRC cells.

Figure 1

(A) The culture medium from an equal number of LS 174T cells expressing the pcDNA3 control plasmid and clones of cells stably expressing L1 (L1 cl1 and cl2), that were kept for 2 days in serum-free medium, was analyzed for the presence of ISG15 by western blotting. (B) The expression of ISG15 RNA was determined by qRT-PCR in the cell clones described in (A). (C) Western blot of the cell clones described in (A) for ISG15 in the cell layer. Ponceau staining was used for determining equal loading and quality control of the western blots. (D) Immunostaining of LS 174T cells stably expressing the pcDNA3 plasmid, or L1, with antibodies to ISG15 (green), L1 (red) and DAPI (blue). The bar represents 10 μm.