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(A) Calibration curve of the absorbance of SiR-en at 630 nm; red marker indicates absorbance of SiR-LDFC-trastuzumab. Absorbance and emission spectra of (B) SiR-en and (C) SiR-LDFC-trastuzumab at a protein concentration of 4 nmol/mL (λEx = 630 nm).
