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. 2019 Dec 24;12:11383–11391. doi: 10.2147/OTT.S235730

Figure 3.

Figure 3

Evo-induced apoptosis was mediated by mitochondria. (A) Cytosolic and mitochondrial cytochrome C expression level in U266 and RPMI8226 after Evo treatment (200, 400 μg/mL) for 24 hrs. (B) Quantification of the western bands of cytosolic and mitochondrial cytochrome C expression level in U266 in (A). (C) Quantification of the western bands of cytosolic and mitochondrial cytochrome C expression level in RPMI8226 in (A). (D) Mitochondrial membrane potential of U266 and RPMI8226 after Evo treatment (200, 400 μg/mL) for 24 hrs. (E) Cells were treated with Evo (200, 400 μg/mL) for 24 hrs with or without 20 μM MitoTEMPO. Cell apoptosis was determined after treatment in two U266 and RPMI8226 cells. (F) ROS production was determined after treatment in two U266 and RPMI8226 cells. *p < 0.05.