Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 24;12:11437–11451. doi: 10.2147/OTT.S223242

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Hu et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

BBM and Gefi synergistically induced PC cell cycle arrest. (A) BBM and Gefi synergistically induce PC cell cycle arrest. Treatment with BBM (5 μM), Gefi (20 μM) alone or in combination with BBM (5 μM) and Gefi (20 μM) for 48 hrs, analysis of cell cycle distribution by cytometry (Becton Dickinson Fascalibor, BD Biosciences, Franklin Lakes, NJ). A typical histogram of cell cycle distribution is given. Data are expressed as the mean ± S.D. of three independent experiments conducted in triplicate. (B) Western blot analysis of cell cycle-related protein Cyclin D1, Cdk4, and Cdk6. Actin was shown as the control of equal loading. (C) The relative expression of cell cycle-related proteins (Cyclin D1/Actin, Cdk4/Actin, and Cdk6/Actin) was quantified by ImageJ software and analyzed by Graphpad prism 7. **P < 0.01 compared to negative control.