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. 2019 Dec 24;12:11437–11451. doi: 10.2147/OTT.S223242

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© 2019 Hu et al.

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BBM and Gefi synergistically induced PC cell apoptosis. (A) Treatment with BBM alone (5 μM), Gefi alone (20 μM) or in combination with BBM (5 μM) and Gefi (20 μM) for 48 hrs, analysis of apoptosis by Annexin V/propidium iodide (PI) staining. Data are expressed as the mean ± S.D. of three independent experiments conducted in triplicate. (B) Total protein was extracted and the expression of Bcl-2, Bax, and Cleaved-PARP was detected by Western blot. Actin was shown as the control of equal loading. (C) The relative expression of apoptosis-related proteins (Cleaved-PARP/Actin, Bax/Actin, and Bcl-2/Actin) was quantified by ImageJ software and analyzed by Graphpad prism 7. **P < 0.01 compared to negative control.