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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Dev Biol. 2019 Sep 14;457(1):57–68. doi: 10.1016/j.ydbio.2019.09.004

Figure 3.

Figure 3.

Has2 cko mutants exhibit reduced shelf area and failure of shelf elevation. (A-D) Histological examination of palatal shelf morphology in E13.5 wild type (A and B) and Has2 cko (C and D) embryos. Blue lines in A and B separating the palatal shelf from the maxilla and dashed red lines demarcating the edge of the palatal shelves for shelf area measurement. (E) Shelf area measurements in E13.5 wild type and Has2 cko embryos. (F) Measurements of the distance between the tips of the palatal shelves in E13.5 wild type and Has2 cko embryos. (G-J) Histological examination of palatal shelf morphology in early E14.5 wild type (G and H) and Has2 cko (I and J) embryos. (K) Shelf area measurements in early E14.5 wild type and Has2 cko embryos. (L) Measurements of the distance between the tips of the palatal shelves in early E14.5 wild type and Has2 cko embryos. (M-P) Histological examination of palatal shelf morphology in E15.5 wild type (M and N) and Has2 cko (O and P) embryos. *, P value<0.05; **, P value<0.01. Scale bar: 500μm.