Figure 7.
Enhanced HA degradation prevents palate closure during palate explant culture. (A and B) Inferior view of the palatal shelves and gap between the palatal shelves in wild type E13.5 palate explants before culture. (C and D) Inferior view of the palatal shelves and gap between the palatal shelves in wild type E13.5 palate explants after 24-hour culture. (E and F) Inferior view of the palatal shelves and gap between the palatal shelves in wild type E13.5 palate explants after 48-hour culture. NORCHLD is used to enhance HA degradation during culture in B, D and F. Arrowhead indicating the closed palate. Dashed red lines demarcating the medial edges of the palatal shelves. Scale bars: 1 mm.