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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Aug 6;1126-1127:121748. doi: 10.1016/j.jchromb.2019.121748

Table 1.

HPLC-MS/MS parameters for analytes and internal standards

Compound Transition
ions (m/z)		 Dwell
(msec)		 DP
(V)		 CE
(V)		 CXP
(V)		 Retention
time (min)		 Period
PE2 396.2 > 378.2 100 25 8 10.5 0.72 1
396.2 > 360.2 100 25 13 10.5 0.72
PE2-d4 400.2 > 382.2 100 25 9 10.5 0.71
400.2 > 364.2 100 25 13 10.5 0.71
PGE2-G 444.3 > 409.3 100 25 11 10.5 1.02 1
444.3 > 391.3 100 25 17 10.5 1.02
PGE2-G-d5 449.3 > 396.3 100 25 17 10.5 1.00
PGE2 351.0 > 271.0 100 −40 −23 −6.0 1.41 1
351.0 > 315.0 100 −40 −15 −6.0 1.41
PGE2-d4 355.0 > 275.0 100 −40 −22 −6.0 1.38
355.0 > 319.0 100 −40 −15 −6.0 1.38
PGD2 351.0 > 271.0 100 −40 −23 −6.0 1.63 1
351.0 > 315.0 100 −40 −15 −6.0 1.65
PGD2-d4 355.0 > 275.0 100 −40 −22 −6.0 1.61
355.0 > 319.0 100 −40 −15 −6.0 1.61
15(S)-HETE-EA 346.2 > 62.0 100 40 35 10 5.28 2
346.2 > 91.0 100 40 38 10 5.28
20-HETE-EA 364.0 > 62.0 100 40 35 10 4.44 2
364.0 > 91.0 100 40 38 10 4.44
5,6-EET-EA 364.0 > 62.0 100 40 35 10 6.85 2
364.0 > 91.0 100 40 38 10 6.85
8,9-EET-EA 364.0 > 62.0 100 40 35 10 6.65 2
364.0 > 91.0 100 40 38 10 6.65
11,12-EET-EA 364.0 > 62.0 100 40 35 10 6.41 2
364.0 > 91.0 100 40 38 10 6.41
14,15-EET-EA 364.0 > 62.0 100 40 35 10 6.09 2
364.0 > 91.0 100 40 38 10 6.09
5,6-DiHETE-EA 364.0 > 62.0 100 40 35 10 4.99 2
364.0 > 91.0 100 40 38 10 4.99
8,9-DiHETE-EA 364.0 > 62.0 100 40 35 10 4.48 2
364.0 > 91.0 100 40 38 10 4.48
11,12-DiHETE-EA 364.0 > 62.0 100 40 35 10 3.91 2
364.0 > 91.0 100 40 38 10 3.91
14,15-DiHETE-EA 364.0 > 62.0 100 40 35 10 3.28 2
364.0 > 91.0 100 40 38 10 3.28
AA 303.2 > 259.2 20 −50 −25 10 9.37 3
303.2 > 59.1 20 −50 −60 10 9.37
AA-d8 311.2 > 267.2 20 −50 −25 10 9.35
311.2 > 59.1 20 −50 −60 10 9.35
AEA 348.2 > 62.2 20 26 13 10 9.01 3
348.2 > 91.2 20 26 60 10 9.01
AEA-d8 356.2 > 62.2 20 26 13 10 9.01
2-AG 379.3 > 287.3 20 60 25 10 9.16 3
378.3 > 269.3 20 60 29 10 9.16
2-AG-d5 384.3 > 287.3 20 60 25 10 9.16
387.3 > 269.4 20 60 29 10 9.16
PEA 300.0 > 62.0 20 31 28 10 9.21 3
300.0 > 283.0 20 31 19 10 9.21
PEA-d4 304.0 > 62.0 20 31 28 10 9.20
OEA 326.0 > 62.0 20 40 30 10 9.29 3
326.0 > 309.0 20 40 20 10 9.29
OEA-d4 330.0 > 66.0 20 40 30 10 9.28

Deprotonation Potential = DP, Collision Energy = CE, Collision Exit Potential = CXP