Figure 4: WRNexo^Δ mutants exhibit altered activity.

Drosophila activity monitor data was collected for young (1-4 day old) and old (28 day old) w¹¹¹⁸ and WRNexo^Δ adults separated by sex over a 6-day period. A) Overall activity is dependent on age and genotype for both male and female flies (Kruskal-Wallis test, p < 0.0001). Dunn’s post showed significant differences between old w¹¹¹⁸ and WRNexo^Δ in both sexes, decreased activity in old males, and increased activity in old females (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). B) Average hourly activity was calculated over a 24-hour day. Activity peaks are evident at light transition periods represented by the black and white bars. The Kolmogorov-Smirnov test showed a significant difference in activity distribution between w¹¹¹⁸ and WRNexo^Δ in old females (p < 0.01). Significant differences in activity at specific hourly intervals were determined using the Kruskal-Wallis test. (*p < 0.01). Data are represented by mean and SEM of single adults (n = 64/sex/genotype).