Table 2.
Oligonucleotide primer sequences used for detection of ESBL genes and PCR cycling conditions used.
| Primer | Primer sequence (5′–3′) | Target gene | Amplicons size (bp) | PCR conditions and cycles | Reference |
|---|---|---|---|---|---|
| blaTEMF | AAACGCTGGTGAAAGTA | blaTEM | 822 | 1 cycle of 5 minutes at 94°C, 35 cycles of 30 seconds at 94°C, 1 minute at 45°C, 1 minute at 72°C; 1 cycle of 10 minutes at 72°C | [27] |
| blaTEMR | AGCGATCTGTCTAT | ||||
| blaSHVF | ATGCGTTATATTCGCCTGTG | blaSHV | 753 | ||
| blaSHVR | TGCTTTGTTATTCGGGCCAA | ||||
| blaCTX-MF | CGCTTTGCGATGTGCAG | blaCTX-M | 550 | ||
| blaCTX-MR | ACCGCGATATCGTTGGT | ||||
| blaOXAF | ATATCTCTACTGTTGCATCTCC | blaOXA | 619 | ||
| blaOXAR | AAACCCTTCAAACCATCC | ||||
| blaCMY-1F | GTGGTGGATGCCAGCATCC | blaCMY-1 | 915 | 1 cycle of 3 minutes at 94°C, 25 cycles of 1 minute at 94°C, 1 minute at 58°C, 1 minute at 72°C; 1 cycle of 10 minutes at 72°C | [26] |
| blaCMY-1R | GGTCGAGCCGGTCTTGTTGAA | ||||
| blaCMY-2F | GCACTTAGCCACCTATACGGCAG | blaCMY-2 | 758 | ||
| blaCMY-2R | GCTTTTCAAGAATGCGCCAGG |