Table 2.
Percentage of healthy, apoptotic, and necrotic cells of 3T3 treated with TQ-NLC as determined by flow cytometry.
| 24 h | 48 h | 72 h | ||
|---|---|---|---|---|
| Control | Healthy cells | 91.7 ± 0.1 | 89.4 ± 0.0 | 86.7 ± 1.2 |
| Early apoptosis | 0.9 ± 0.1 | 1.2 ± 0.1 | 2.3 ± 0.6 | |
| Late apoptosis | 6.6 ± 0.1 | 8.7 ± 0.1 | 9.9 ± 0.6 | |
| Necrosis | 0.8 ± 0.1 | 0.7 ± 0.0 | 1.1 ± 0.1 | |
|
| ||||
| NLC | Healthy cells | 90.1 ± 0.2 | 89.9 ± 0.1 | 87.4 ± 0.0 |
| Early apoptosis | 2.0 ± 0.1 | 2.2 ± 0.2 | 2.7 ± 0.3 | |
| Late apoptosis | 4.6 ± 0.1 | 4.5 ± 0.2 | 5.8 ± 0.3 | |
| Necrosis | 3.3 ± 0.1 | 3.4 ± 0.4 | 4.1 ± 0.1 | |
|
| ||||
| 3 μM | Healthy cells | 92.1 ± 0.8 | 89.3 ± 1.5 | 95.1 ± 0.8 |
| Early apoptosis | 1.5 ± 0.3 | 0.9 ± 0.2 | 1.3 ± 0.2 | |
| Late apoptosis | 5.0 ± 0.6 | 8.3 ± 1.2 | 2.7 ± 0.4 | |
| Necrosis | 1.5 ± 0.4 | 1.5 ± 0.5 | 0.9 ± 0.2 | |
|
| ||||
| 6 μM | Healthy cells | 89.9 ± 0.2 | 92.8 ± 0.1 | 93.6 ± 0.9 |
| Early apoptosis | 0.9 ± 0.1 | 0.6 ± 0.1 | 1.8 ± 0.1 | |
| Late apoptosis | 7.7 ± 0.3 | 4.9 ± 0.2 | 3.9 ± 0.1 | |
| Necrosis | 1.5 ± 0.0 | 1.8 ± 0.4 | 0.7 ± 0.7 | |
|
| ||||
| 12 μM | Healthy cells | 93.6 ± 2.0 | 94.7 ± 0.2 | 93.2 ± 0.7 |
| Early apoptosis | 0.2 ± 1.1 | 0.5 ± 0.1 | 1.1 ± 0.1 | |
| Late apoptosis | 0.8 ± 2.4 | 3.8 ± 0.0 | 4.4 ± 0.6 | |
| Necrosis | 5.4 ± 1.5 | 1.0 ± 0.0 | 1.3 ± 0.0 | |
Each data point represents the mean of three independent experiments. Each value was represented as mean ± SEM.