F. heteroclitus embryos were treated from 1 to 7 dpf with DMSO (0.01%, vehicle), TBT (Low: 5 nM, Medium: 50 nM and High: 100 nM) in seawater and then moved to a filter paper moistened with clean water until 10 dpf. (a) Representative images of caudal fin development. (b) Caudal fin deformity score for 10 dpf embryos. Deformity score ranged from 0 to 3, with 0 = normal and 3 = severe deformity. Data are presented as mean ± SEM. Total N≥50, screened from two replicate experiments. Two Factor ANOVA: Population p = 0.435, Treatment p <0.0001, Interaction p = 0.483. * Significantly different from DMSO in the same population (p<0.05, ANOVA, Dunnett’s)