F. heteroclitus embryos were treated as described in Figure 3. mRNA expression was measured by RT-qPCR in pooled embryonic killifish. (a) Expression of pparg. Two Factor ANOVA: Population, p = 0.004. Treatment p = 0.004. Interaction p = 0.364. (b) Expression of fabp1b. Two Factor ANOVA: Population p = 0.036, Treatment p = 0.363, Interaction: p = 0.407. Each pool consisted of 5 embryos. N=6 pools per chemical treatment, per population from three experimental replicates from SC and NBH. Data points of the same color are from biological replicates produced in the same experiment. * Significantly different from DMSO in the same population (p<0.05, ANOVA, Dunnett’s)