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. 2019 Dec 23;7:304. doi: 10.3389/fcell.2019.00304

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Copyright © 2019 Makowiecka, Malek, Mazurkiewicz, Mrówczyńska, Nowak and Mazur.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thymosin β4 is a constituent of focal adhesions (FAs) and forms complexes with FAs constituents in WM1341D and A375 cell lines. (A) Schematic representation of DNA constructs used in this study. (B) Immunocytochemical stainings visualizing FAs components: vinculin and αVβ3 integrin and co-localizing with them Tβs. (C) Visualization of vinculin and exogenous HA-Tβ4 in transfected cells. (D) PLA of HA-Tβ4 vs. vinculin, ILK, FAK, Pinch 2, α parvin, and αVβ3 integrin in transfected cells. Cell nuclei are shown in dark gray. Arrows point at FAs, while red arrowheads indicate PLA complexes.