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. 2019 Dec 23;7:304. doi: 10.3389/fcell.2019.00304

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Copyright © 2019 Makowiecka, Malek, Mazurkiewicz, Mrówczyńska, Nowak and Mazur.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Focal adhesion formation in WM1341D and A375 cells and effect of TMSB4X expression silencing on formation of FAs in melanoma cells. (A,F) Immunocytochemical analysis of cells in order to visualize vinculin. Cells’ borders were marked for better orientation with white lines. Quantitation of FAs’ number (B,G), size of FA’s (C,H), and cell’s area (D,I) (n = 20). (E,J) Adhesion assays of investigated cell lines and clones (n = 3). Arrows point at FAs. The significance level was set at ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001 (www.oncomine.org, February 2018, Thermo Fisher Scientific, Ann Arbor, MI, United States).