FIGURE 5.

Decreasing of Tβ4 level affects A375 cells’ 2D motility. The cells were seeded either on non-coated surface (A–E) or on Matrigel^TM surface (F–J). (A–C,F–H) Spontaneous 2D-migration of tested cells. The cells were recorded over 72 h (n = 30): (A,F) trajectories of single cells migration, (B,G) calculated migrated distances of the cells, (C,H) estimation of directionality of cells’ migration. Wound healing assay performed on tested cells (n = 3): (D,I) representative pictures of cells migrating collectively and (E,J) graphs representing scratch closure expressed in % of closed scratch. The significance level was set at ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001 (www.oncomine.org, February 2018, Thermo Fisher Scientific, Ann Arbor, MI, United States).