Table 6.
Linearity, LOD, and LOQ of the four standard compounds.
| Compounds | t R (min) | Equation (linear model)a | Linear range (mg/mL) | r 2b | LODc (μg/mL) | LOQd (μg/mL) |
|---|---|---|---|---|---|---|
| 1 | 9.65 | y = 10,999,847x + 12,569 | 0.001–0.4 | 0.9998 | 0.2507 | 0.7597 |
| 5 | 14.20 | y = 15,200,231x − 3,254 | 1.000 | 0.5774 | 1.7497 | |
| 6 | 14.49 | y = 18,268,572x − 7,852 | 1.000 | 0.5259 | 1.5937 | |
| 7 | 18.81 | y = 28,501,213x + 12,569 | 1.000 | 0.1714 | 0.5195 |
a y: peak area at 207 nm; x: standard concentration (mg/mL). br2: coefficient of determination with 11 indicated points on the calibration curves. cLOD: limit of detection, S/N = 3.3 (n = 7). dLOQ: limit of quantification, S/N = 10 (n = 7).