TABLE 1.
Virulence factors which were significantly upregulated during vaginal colonizationa
| Category | Locus tag | Gene | Fold change by time postinoculation |
||
|---|---|---|---|---|---|
| 5 h | 1 day | 3 days | |||
| Transcriptional regulation | SAUSA300_0195 | Transcriptional regulator | 3 | 3 | 3 |
| SAUSA300_0218 | Sensor histidine kinase family protein | 3 | 3 | 4 | |
| SAUSA300_0255 | Two-component system response regulator | 6 | 7 | 6 | |
| SAUSA300_0878 | LysR family transcriptional regulator | 9 | 5 | 5 | |
| SAUSA300_1220 | LuxR family DNA-binding response regulator | 2 | 2 | 2 | |
| SAUSA300_1257 | msrR | 5 | 5 | 4 | |
| SAUSA300_1514 | fur | 3 | 3 | 2 | |
| SAUSA300_1717 | arsR | 7 | 4 | 5 | |
| SAUSA300_1798 | DNA-binding response regulator | 4 | 4 | 4 | |
| SAUSA300_1799 | Putative sensor histidine kinase | 3 | 4 | 4 | |
| SAUSA300_2098 | Transcriptional repressor | 15 | 13 | 15 | |
| SAUSA300_2300 | TetR family transcriptional regulator | 3 | 3 | 3 | |
| SAUSA300_2322 | TetR family transcriptional regulator | 4 | 4 | 4 | |
| SAUSA300_2336 | MerR family transcriptional regulator | 3 | 2 | 3 | |
| SAUSA300_2347 | nirR | 12 | 8 | 7 | |
| SAUSA300_2437 | sarT | 11 | 18 | 13 | |
| SAUSA300_2566 | arcR | 4 | 4 | 6 | |
| SAUSA300_2571 | argR | 3 | 4 | 6 | |
| SAUSA300_2640 | Putative transcriptional regulator | 5 | 9 | 11 | |
| Toxins | SAUSA300_0800 | sek | 6 | 7 | 7 |
| SAUSA300_0801 | seq | 4 | 6 | 5 | |
| SAUSA300_1058 | hla | 19 | 6 | 18 | |
| SAUSA300_1918 | Truncated beta-hemolysin | 14 | 2 | 9 | |
| Secreted enzymes | SAUSA300_0923 | htrA | 3 | 4 | 4 |
| SAUSA300_0951 | sspA | 2 | 12 | 13 | |
| SAUSA300_1753 | spIF | 12 | 3 | 24 | |
| SAUSA300_1755 | spID | 13 | 5 | 28 | |
| SAUSA300_1756 | spIC | 11 | 3 | 22 | |
| SAUSA300_1757 | spIB | 12 | 4 | 21 | |
| SAUSA300_2572 | aur | 4 | 15 | 12 | |
| ECM binding | SAUSA300_0546 | sdrC | 6 | 12 | 16 |
| SAUSA300_0547 | sdrD | 9 | 19 | 22 | |
| SAUSA300_0774 | empbp | 35 | 4 | 8 | |
| Immune modulation | SAUSA300_1059 | Superantigen-like protein | 10 | 3 | 4 |
| SAUSA300_1060 | Superantigen-like protein | 12 | 4 | 6 | |
| SAUSA300_1061 | Superantigen-like protein | 8 | 6 | 7 | |
| SAUSA300_1920 | chs | 29 | 3 | 8 | |
| SAUSA300_0224b | coa | 4 | |||
| SAUSA300_0836b | dltB | 2 | |||
| SAUSA300_0837b | dltC | 2 | |||
| SAUSA300_1053b | Formyl peptide receptor-like 1 inhibitory protein | 4 | |||
| SAUSA300_1055b | efb | 2 | |||
| SAUSA300_2364b | sbi | 2 | |||
| Type 7 secretion system | SAUSA300_0279b | esaA | 5 | ||
| SAUSA300_0280b | essA | 2 | |||
| SAUSA300_0281b | esaB | 4 | 4 | ||
| SAUSA300_0282 | essB | 2 | 6 | 6 | |
| SAUSA300_0283b | essC | 3 | 3 | ||
| SAUSA300_0284b | esxC | 3 | 2 | ||
| SAUSA300_0286b | essE | 3 | 3 | ||
| SAUSA300_0287b | esxD | 3 | 3 | ||
| SAUSA300_0288b | essD | 2 | |||
| SAUSA300_0290 | DUF5079 family protein | 6 | 9 | 9 | |
| SAUSA300_0291 | DUF5080 family protein | 5 | 7 | 6 | |
| SAUSA300_0298 | essI5 | 3 | 4 | 3 | |
| SAUSA300_0299b | essI6 | 2 | |||
| SAUSA300_0300b | essI7 | 2 | 2 | ||
| SAUSA300_0302 | essI9 | 3 | 5 | 5 | |
| SAUSA300_0303b | DUF4467 domain-containing protein | 2 | 3 | ||
a
Genes which encode transcriptional regulators, toxins, secreted enzymes, extracellular matrix (ECM) binding, immune modulation, and T7SS factors and that were differentially expressed in mouse swab samples at all three time points are listed with their respective fold changes relative to growth in TSB.
b
Genes which were not significantly differentially expressed at all time points.