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. Author manuscript; available in PMC: 2020 May 21.
Published in final edited form as: Anal Chem. 2019 May 9;91(10):6808–6814. doi: 10.1021/acs.analchem.9b00981

Figure 2.

Figure 2.

Weighted average charge state as a function of solution temperature with midpoint melting temperature Tm = 67.5 ± 2.2 °C, and a second transition at Tm2 = 87.1 ± 3.0 °C. Inset mass spectra show shifts in charge state, and dissociation of the cofactor from the holoprotein to form the apo state with increasing temperature. Filled circles represent the holoprotein, and open circles denote the apoprotein. A table of all observed m/z values is provided in the Supporting Information (Table S1).