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. 2019 Dec 23;58(1):e00907-19. doi: 10.1128/JCM.00907-19

TABLE 1.

Reference Tm values with standard deviations and Tm windows as defined by the Ultra assaya

rpoB genotype Mean
or
SD		 Value (°C) for Tm windows as defined by the Ultra assay for:
rpo1 rpo1
Mut		 rpo2 rpo2
Mut		 rpo3 rpo3
Mut		 rpo4 rpo4
MutA		 rpo4
MutB
Genotypes with one set of reference Tm values
    WT Mean 69.57 0 73.29 0 75.96 0 67.27 0 0
SD 0.16 0 0.16 0 0.22 0 0.15 0 0
    511CCG Mean 0 63.52 73.22 0 75.93 0 67.28 0 0
SD 0 0.05 0.05 0 0.05 0 0.05 0 0
    513CAGb Mean 68.17 0 75.1 0 75.93 0 67.47 0 0
SD 0.06 0 0.17 0 0.21 0 0.15 0 0
    516GTC Mean 70.06 0 0 69.72 75.81 0 67.42 0 0
SD 0.12 0 0 0.1 0.11 0 0.18 0 0
    516TAC Mean 69.71 0 0 68.91 75.7 0 67.39 0 0
SD 0.12 0 0 0.17 0.14 0 0.13 0 0
    522CAG Mean 70.12 0 0 68.83 0 73.95 67.12 0 0
SD 0.1 0 0 0.1 0 0.06 0.05 0 0
    522TTG Mean 69.6 0 0 70.2 0 73.66 67.26 0 0
SD 0.07 0 0 0.07 0 0.09 0.05 0 0
    526CCC Mean 69.47 0 73.45 0 0 71.7 66.08 0 0
SD 0.05 0 0.06 0 0 0 0.1 0 0
    526TAA Mean 69.27 0 73.17 0 0 71.7 67.57 0 0
SD 0.06 0 0.12 0 0 0.1 0.06 0 0
    526TAC Mean 69.44 0 73.49 0 0 72.83 67.29 0 0
SD 0.1 0 0.13 0 0 0.13 0.16 0 0
    526TCC Mean 69.37 0 73.37 0 0 70.63 67.3 0 0
SD 0.06 0 0.15 0 0 0.12 0.1 0 0
    526TGC Mean 69.4 0 73.43 0 0 71.7 68.43 0 0
SD 0.1 0 0.12 0 0 0.1 0.06 0 0
    531CAG Mean 69.27 0 73.33 0 0 71.23 67.1 0 0
SD 0.06 0 0.06 0 0 0.06 0 0 0
    531TGG Mean 69.62 0 73.78 0 0 73.54 0 71.06 0
SD 0.08 0 0.08 0 0 0.09 0 0.05 0
    531TTG Mean 69.5 0 74.27 0 0 73.44 0 73.89 0
SD 0.12 0 0.16 0 0 0.19 0 0.13 0
    533CCG Mean 69.64 0 73.3 0 75.98 0 0 0 61.68
SD 0.13 0 0.12 0 0.1 0 0 0 0.13
    529AAAc Mean 69.53 0 72.98 0 0 0 0 0 64.08
SD 0.05 0 0.08 0 0 0 0 0 0.08
    518AAC del Mean 69.53 0 0 65.73 75.5 0 66.93 0 0
SD 0.21 0 0 0.38 0.26 0 0.06 0 0
    510CTG_516TAC Mean 0 65.73 0 69.07 75.77 0 67.47 0 0
SD 0 0.06 0 0.12 0.12 0 0.06 0 0
    511CCG_516GGC Mean 0 65.9 71.87 0 75.83 0 67.47 0 0
SD 0 0.1 0.15 0 0.12 0 0.06 0 0
    512CGC_526AAC Mean 0 63.7 73.67 0 0 72.53 67.53 0 0
SD 0 0.1 0.12 0 0 0.15 0.06 0 0
    512GGC_531TGG Mean 0 66.75 74.05 0 0 73.65 0 71.1 0
SD 0 0.06 0.06 0 0 0.13 0 0.08 0
    513CTA_523GAG Mean 0 0 71.55 0 0 72.28 67.08 0 0
SD 0 0 0.06 0 0 0.05 0.13 0 0
    513CTA_526TAA Mean 0 0 73.23 0 0 71.8 67.7 0 0
SD 0 0 0.15 0 0 0.1 0.1 0 0
    515ATT_516TAC Mean 69.87 0 0 68.74 75.81 0 67.2 0 0
SD 0.17 0 0 0.19 0.12 0 0.1 0 0
    515ATT_526AAC Mean 69.6 0 0 70.03 0 72.5 67.17 0 0
SD 0.1 0 0 0.06 0 0 0.12 0 0
    516GGC_533CCG Mean 70.85 0 71.77 0 75.78 0 0 0 61.62
SD 0.1 0 0.05 0 0.08 0 0 0 0.13
    516TAC_531TTG Mean 69.8 0 0 68.5 0 73.23 0 73.8 0
SD 0.1 0 0 0.1 0 0.06 0 0.1 0
    522TTG_531GCG Mean 69.57 0 0 70.3 0 72.87 67.7 0 0
SD 0.31 0 0 0.1 0 0.12 0.1 0 0
    530ATG_531TTC Mean 69.5 0 73.63 0 0 69.53 0 0 64
SD 0.17 0 0.21 0 0 0.12 0 0 0.1
    512ACC_515ATT_526AAC Mean 0 61.62 0 69.9 0 72.53 67.48 0 0
SD 0 0.04 0 0.06 0 0.08 0.1 0 0
Genotypes with two sets of reference Tm values
    526AGC-1 Mean 69.4 0 73.5 0 0 71.5 68.9 0 0
SD NA NA NA NA NA NA NA NA NA
    526AGC-2c Mean 69.53 0 73.61 0 0 71.59 0 0 0
SD 0.11 0 0.18 0 0 0.13 0 0 0
    526CGC-1 Mean 69.51 0 73.46 0 0 74.25 0 69.36 0
SD 0.03 0 0.05 0 0 0.05 0 0.07 0
    526CGC-2c Mean 69.55 0 73.49 0 0 0 0 69.39 0
SD 0.08 0 0.08 0 0 0 0 0.09 0
    509AGG_526CGC-1 Mean 0 66.1 73.47 0 0 74.27 0 69.43 0
SD 0 0.15 0.05 0 0 0.05 0 0.1 0
    509AGG_526CGC-2c Mean 0 66.09 73.55 0 0 0 0 69.3 0
SD 0 0.14 0.14 0 0 0 0 0.21 0
Genotype with three sets of reference Tm values
    518GAC_533CCG-1c Mean 69.42 0 0 0 75.77 0 0 0 61.45
SD 0.13 0 0 0 0.12 0 0 0 0.14
    518GAC_533CCG-2 Mean 69.6 0 71.5 0 75.9 0 0 0 61.7
SD NA NA NA NA NA NA NA NA NA
    518GAC_533CCG-3 Mean 69.2 0 0 71.1 75.6 0 0 0 61.3
SD NA NA NA NA NA NA NA NA NA
Grouped genotypes
    526AAC or 526CTC Mean 69.38 0 73.29 0 0 72.41 67.47 0 0
SD 0.13 0 0.1 0 0 0.06 0.09 0 0
    526GAC or 531TTC Mean 69.42 0 73.56 0 0 72.19 68.15 0 0
SD 0.17 0 0.23 0 0 0.21 0.15 0 0
    516GAC del or 516GTC_518GAC Mean 69.65 0 0 66.75 75.75 0 67.14 0 0
SD 0.09 0 0 0.13 0.09 0 0.11 0 0
a

n = 9 Tm windows. SD information was unavailable for 3 references because the cases are too rare to provide meaningful SD data.

b

Silent mutation.

c

References with only 3 positive Tm values.