TABLE 1.

Primers used to amplify each gene in the MLST schemes

Gene locus	Primers, sequences (5′–3′)	Size (bp)	Analyzed fragment (5′–3′)	Reference
hsp65	HSP65-F, ACCAACGATGGTGTGTCCAT; HSP65-R, CTTGTCGAACCGCATACCCT	401	CGCCAAGGAG…GAGCTCACCG	21
rpoB	RPOB-F, GGCAAGGTCACCCCGAAGGG; RPOB-R, AGCGGCTGCTGGGTGATCATC	711	TGARACCGAG…GBCCGTACTC	21
secA1	SECA1-F, GACAGYGAGTGGATGGGYCGSGTGCACCG; SECA1-R, GCGGACGATGTARTCCTTGTCSCG	466	CTTCCTVGGS…RCTRTTCMMS	21
argH	ARGHF, GACGAGGGCGACAGCTTC; ARGHSR1, GTGCGCGAGCAGATGATG	480	GTGAGCACYAACGAAGGCTC…CGATCATGCCGGGCAAGACT	11
cya	ACF, GTGAAGCGGGCCAAGAAG; ACSR1, AACTGGGAGGCCAGGAGC	510	CTGGTGGGGTCCACCCAGTT…TKGCGCGCCCGCGTCACGGC	11
glpK	GLPKSF1, AATCTCACCGGCGGTGTC; GLPKSR2, GGACAGACCCACGATGGC	534	GTGACAAATGCCAGTCGCAC…TGTTCGCGCCGTACTGGCGR	11
gnd	GNDF, GTGACGTCGGAGTGGTTGG; GNDSR1, CTTCGCCTCAGGTCAGCTC	480	CARTTCRTTGAAGAYGTGCG…WCCGYAACGAAGTWGAGGCG	11
murC	MURCSF1, CGGACGAAAGCGACGGCT; MURCSR2, CCAAAACCCTGCTGAGCC	537	CCGAACCTGATCRTCGTSAC…AGGTGCGYACRGTGCTGCAG	11
pta	PTASF1, GATCGGGCGTCATGCCCT; PTASR2, ACGAGGCACTGCTCTCCC	486	GACGTMCTACTSGCCGTGGC…AAATCCGYTCCCGTGCYGCC	11
purH	PURHSF1, CGGAGGCTTCACCCTGGA; PURHSR2, CAGGCCACCGCTGATCTG	549	AAGGTTYTRGCTGCCAAGGC…GCAAGAAGAACGTGCGGCTG	11
gdhA	GDHAF, GTCAGTGCCCCGATCGCTGDHASR1, GGCTCTCGGAGTACGTCGA	542	GTCGACGGGDCMGAAGGGTC…GAGCTCCCCCGCCGTGTTYT	7
pgm	PGMSF1, CCATTTGAACCCGACCGG; PGMSR2, GTGCCAACGAGATCCTGCG	559	TACCTCGATCAGCGTCCGGC…TCACCGAGCGCCAGCCGTCG	7
pknA	PKNAF, CAGGTGGACCTCGGACATG; PKNASR1, AACCAGGCGCCCACCATC	457	CCGCCATAGCCGAGGATCTC…GCAGCCGGCGTCGCSCGGCT	7