TABLE 3.
Comparison of three-, seven-, and thirteen-gene MLST schemes for 139 M. abscessus isolates
| Subspecies (n) | Subspecies and sequence type detection (n) |
||
|---|---|---|---|
| Three-gene MLST | Seven-gene MLST | Thirteen-gene MLST | |
| M. abscessus subsp. abscessus (54) | MAB1 (40) | ST1 (22) | ST1 (22) |
| ST22 (1) | ST22 (1) | ||
| ST40 (1) | ST40 (1) | ||
| ST63 (3) | ST63 (3) | ||
| ST127 (5) | ST127 (5) | ||
| ST276c (1) | ST276c (1) | ||
| ST280c (1) | ST280c (1) | ||
| ST272c (1) | ST272s (1) | ||
| ST289c (1) | ST289c (1) | ||
| ST277c (1) | ST277c (1) | ||
| ST283c (1) | ST283c (1) | ||
| ST284c (1) | ST284c (1) | ||
| ST288c (1) | ST288c (1) | ||
| MAB2 (2) | ST126 (2) | ST126 (2) | |
| MAB3 (4) | ST33 (1) | ST33 (1) | |
| ST49 (2) | ST49 (2) | ||
| ST286c (1) | ST286c (1) | ||
| MAB4 (2) | ST137 (1) | ST137 (1) | |
| ST278c (1) | ST278c (1) | ||
| MAB5 (1) | ST1a (1) | ST1aa (1) | |
| MAB6 (1) | ST61 (1) | ST61 (1) | |
| MAB7 (1) | ST282c (1) | ST282c (1) | |
| MAB8 (1) | ST281c (1) | ST281c (1) | |
| MAB9 (1) | ST290c (1) | ST290c (1) | |
| MAB10 (1) | ST274c (1) | ST274c (1) | |
| M. abscessus subsp. massiliense (85) | MMA1b (57) | ST48b (57) | ST48b (57) |
| MMA2 (11) | ST117 (10) | ST117 (10) | |
| ST275c (1) | ST275c (1) | ||
| MMA3 (1) | ST271c (1) | ST271c (1) | |
| MMA4 (4) | ST23 (4) | ST23 (4) | |
| MMA5 (3) | ST176 (2) | ST176 (2) | |
| ST287c (1) | ST287c (1) | ||
| MMA6 (3) | ST115 (2) | ST115 (2) | |
| ST285c (1) | ST285c (1) | ||
| MMA7 (1) | ST279c (1) | ST279c (1) | |
| MMA8 (1) | ST34 (1) | ST34 (1) | |
| MMA9 (1) | ST273c (1) | ST273c (1) | |
| MMA10 (1) | ST291c (1) | ST291c (1) | |
| MMA11 (1) | ST279a ,c (1) | ST279aa (1) | |
| MMA12 (1) | ST37 (1) | ST37 (1) | |
a
Denotes where typing results obtained by seven- and thirteen-gene MLST were not in agreement.
b
For MMA1/ST48, all 57 outbreak isolates shared the same PFGE and rep-PCR patterns. Of the 57 isolates, 51 were obtained from contaminated ultrasonography or patients with documented exposure to invasive procedures following ultrasonography. Two isolates were from hospital water supplying two bronchoscopic units, three were from three patients’ sputum, and one was from a wound without documented exposure to contaminated ultrasonography gel.
c
New STs from this study that were submitted to the Pasteur Institute and assigned a new sequence number (ST271 to ST291).